| VfR Aalen beat Holstein Kiel 1-0 (H) on 6 Aug 2016 |
| Holstein Kiel beat Sportfreunde Lotte 3-1 (H) on 9 Aug 2016 |
| Sportfreunde Lotte beat Hansa Rostock 3-1 (A) on 17 Sep 2016 |
| Hansa Rostock beat Fortuna Köln 2-0 (A) on 21 Sep 2016 |
| Fortuna Köln beat Chemnitzer 1-0 (H) on 10 Sep 2016 |
| Chemnitzer beat FSV Zwickau 1-0 (H) on 16 Sep 2016 |
| FSV Zwickau beat Jahn Regensburg 4-0 (H) on 20 Sep 2016 |
| Jahn Regensburg beat Sonnenhof Großaspach 4-3 (A) on 7 Aug 2016 |
| Sonnenhof Großaspach beat Hallescher 3-0 (H) on 27 Aug 2016 |
| Hallescher beat Preußen Münster 2-1 (H) on 9 Sep 2016 |
| Preußen Münster beat Mainz II 1-0 (H) on 27 Aug 2016 |
| Mainz II beat Magdeburg 1-0 (H) on 10 Sep 2016 |
| Magdeburg beat Werder Bremen II 2-0 (H) on 21 Sep 2016 |
| Werder Bremen II beat VfL Osnabrück 4-2 (H) on 26 Aug 2016 |
| VfL Osnabrück beat Wehen Wiesbaden 1-0 (H) on 9 Sep 2016 |
| Wehen Wiesbaden beat MSV Duisburg 3-0 (H) on 16 Sep 2016 |
| MSV Duisburg beat Paderborn 1-0 (H) on 29 Jul 2016 |
| Paderborn beat Rot Weiß Erfurt 3-1 (A) on 17 Sep 2016 |
| Rot Weiß Erfurt beat FSV Frankfurt 1-0 (A) on 6 Aug 2016 |
FLP: Thick arrows show the earliest chain of wins that passes through every team.